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The apical ectodermal ridge expresses high amounts or the homeobox gene GHox-8 when placed upon dissociated limb mesenchymal cells in culture and induces high expression of GHox-7, but only low expression of GHox-8, in the underlying mesenchymal cells. Ectoderm from the proximal anterior border of the limb induces high expression of both GHox-7 and GHox-8, while ectoderm from the proximal posterior border does not induce expression of either gene. Thus, ectoderm in various regions of the limb bud has distinct regulatory activities and may be involved in controlling the regionally specific expression of GHox-7 and Beats Dr Dre Studio Canada GHox-8 in the mesoderm. Two tissue culture methods and 5 cell lines Beats Pro Best Buy Canada were compared in measuring the values of cytotoxicity 50 per cent (CT50) and CT0 of 40 different drugs and 25 diverse chemical compounds. The CT50 values obtained under each cell culture technique used did not show significant differences.There were marked differences in cytotoxicity and dose responses of the chemical compounds could be assigned to 3 categories, and the compounds tested were differentiated into 3 classes. The importance of the dose response to studies in viral chemotherapy is stressed, and the ability of CT50 and CT0 in other fields of biomedical research is discussed.